Wasserman T H, Twentyman P
MRC Unit University Department of Clinical Oncology and Radiotherapeutics, Cambridge, England.
Int J Radiat Oncol Biol Phys. 1988 Sep;15(3):699-702. doi: 10.1016/0360-3016(88)90314-8.
We assessed the use of a colorimetric assay for determination of radiosensitivity for cells taken directly from murine solid tumors. The assay uses microtier plates and measures the ability of viable cells to reduce a tetrazolium salt (MTT) to an insoluble form, a formazan salt. We established the dependency of the assay on the cell number and time of assay for two murine tumors (EMT-6 and RIF-1). We compared the MTT assay to the standard clonogenic assay and had good agreement of surviving fraction after radiation doses of 2 and 4 Gy. It is possible, therefore, to adapt the MTT assay for use with cell suspensions prepared directly from fresh murine tumors. This may provide a methodology for the determination of the clinical radiosensitivity of tumors including fresh clinical tumor specimens.
我们评估了一种比色测定法在直接取自小鼠实体瘤的细胞放射敏感性测定中的应用。该测定法使用微量滴定板，测量活细胞将四唑盐（MTT）还原为不溶性形式即甲臜盐的能力。我们确定了该测定法对两种小鼠肿瘤（EMT - 6和RIF - 1）的细胞数量和测定时间的依赖性。我们将MTT测定法与标准克隆形成测定法进行了比较，在2 Gy和4 Gy辐射剂量后存活分数具有良好的一致性。因此，有可能将MTT测定法适用于直接从新鲜小鼠肿瘤制备的细胞悬液。这可能为包括新鲜临床肿瘤标本在内的肿瘤临床放射敏感性测定提供一种方法。
