通过直接从血液中捕获快速检测丙型肝炎病毒RNA

Rapid detection of hepatitis C virus RNA by direct capture from blood.

作者信息

van Doorn L J, Kleter B, Voermans J, Maertens G, Brouwer H, Heijtink R, Quint W

机构信息

Diagnostic Centre SSDZ, Department of Molecular Biology, Delft, The Netherlands.

出版信息

J Med Virol. 1994 Jan;42(1):22-8. doi: 10.1002/jmv.1890420105.

DOI:10.1002/jmv.1890420105

PMID:8308516

Abstract

A new diagnostic assay for hepatitis C virus RNA detection is described. HCV genomic RNA is captured onto streptavidin-coated magnetic beads by solution hybridization with biotinylated complementary oligonucleotides. The specificity of the capture assay is confirmed using different capture oligonucleotides as well as sera representing different types of HCV. Sensitivity was determined by testing serial dilutions of a HCV infected plasma. A panel of 50 sera was tested for anti-HCV by a Line Immunoassay and for HCV-RNA by both a conventional guanidinium extraction method and the new capture assay. The specificity of the capture assay was 95.8% and the sensitivity was 92.3% compared to the standard protocol. This method provides a rapid and simple alternative for HCV-RNA detection in blood samples.

摘要

本文描述了一种用于丙型肝炎病毒RNA检测的新诊断方法。通过与生物素化的互补寡核苷酸进行溶液杂交，将HCV基因组RNA捕获到链霉亲和素包被的磁珠上。使用不同的捕获寡核苷酸以及代表不同类型HCV的血清来确认捕获检测的特异性。通过检测HCV感染血浆的系列稀释液来确定灵敏度。通过线免疫分析检测了一组50份血清中的抗HCV，并通过传统的胍提取方法和新的捕获检测方法检测了HCV-RNA。与标准方案相比，捕获检测的特异性为95.8%，灵敏度为92.3%。该方法为血液样本中HCV-RNA的检测提供了一种快速简便的替代方法。