Tachezy R, Hamsikova E, Valvoda J, Van Ranst M, Betka J, Burk R D, Vonka V
Department of Experimental Virology, First Medical Faculty, Charles University, Prague, Czechoslovakia.
J Med Virol. 1994 Jan;42(1):52-9. doi: 10.1002/jmv.1890420111.
Recurrent respiratory papillomatosis (RRP) is the most common benign tumour of the larynx, affecting both children and adults. We present a series of 25 patients, including 10 cases of juvenile multiple, 8 cases of adult solitary, and 7 cases of adult multiple RRP. Biopsy tissue from each patient was screened by Southern blot hybridization and polymerase chain reaction for the presence of human papillomavirus (HPV) DNA. Sera from patients and age- and sex-matched controls were tested for the presence of HPV-specific antibodies using a synthetic peptide derived from the minor capsid protein (L2) of HPV 6/11. By Southern blot hybridization and/or polymerase chain reaction, biopsies from all patients were positive for HPV 6/11 DNA. There was no difference in antibody response between cases and controls. Female cases and controls had significantly higher antibody titers than male subjects. A correlation was observed between the HPV-specific antibody level and the number of surgery-necessitating recurrences.
复发性呼吸道乳头状瘤病（RRP）是最常见的喉部良性肿瘤，可累及儿童和成人。我们报告了25例患者，包括10例青少年多发性、8例成人单发性和7例成人多发性RRP。通过Southern印迹杂交和聚合酶链反应对每位患者的活检组织进行筛查，以检测人乳头瘤病毒（HPV）DNA的存在。使用源自HPV 6/11次要衣壳蛋白（L2）的合成肽，检测患者以及年龄和性别匹配的对照血清中HPV特异性抗体的存在。通过Southern印迹杂交和/或聚合酶链反应，所有患者的活检组织HPV 6/11 DNA均呈阳性。病例组和对照组之间的抗体反应没有差异。女性病例组和对照组的抗体滴度显著高于男性受试者。观察到HPV特异性抗体水平与需要手术的复发次数之间存在相关性。
