Sameshima A, Fujiyoshi T, Pholampaisathit S, Ushikai M, Kono M, Antarasena S, Fukuda K, Furuta S, Sonoda S, Ohyama M
Department of Otolaryngology, Faculty of Medicine, Kagoshima University, Japan.
Auris Nasus Larynx. 1997 Apr;24(2):185-91. doi: 10.1016/s0385-8146(96)00000-4.
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand, with the human papillomavirus (HPV) type-11 being the most widespread. In this study, we isolated the HPV type-11 (HPV-11) genome from subjects with RRP and subcloned the E6 and L2 open reading frames (ORFs) with the expression vectors pEX1 and pEX3. The recombinant E6/beta-gal and L2/beta-gal fusion proteins were expressed in E. coli. Using the recombinant proteins, we demonstrated the presence of antibodies against HPV-11 E6 and L2 in RRP patients by Western blot analysis. The prevalence of seropositivity for HPV-11 E6 and L2 were 5% (1/20) and 10% (2/20), respectively. Although RRP is caused by infection on the mucosal surface, it appears that an immune response occurs against viral proteins expressed in the epithelial lesions.
