泰国复发性呼吸道乳头状瘤病相关人乳头瘤病毒DNA的检测与克隆
Detection and cloning of human papillomavirus DNA associated with recurrent respiratory papillomatosis in Thailand.
作者信息
Ushikai M, Fujiyoshi T, Kono M, Antrasena S, Oda H, Yoshida H, Fukuda K, Furuta S, Hakura A, Sonoda S
机构信息
Department of Otolaryngology, Faculty of Medicine, Kagoshima University.
出版信息
Jpn J Cancer Res. 1994 Jul;85(7):699-703. doi: 10.1111/j.1349-7006.1994.tb02417.x.
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand. In this study, we examined the presence of human papillomavirus (HPV) DNA in 25 RRP patients in Thailand by means of dot blot analysis and/or polymerase chain reaction. Eighty-four percent (21/25) of cases and 4% (1/25) of cases were positive for HPV-11 DNA and HPV-6 DNA, respectively. Three cases (3/25) were negative for all of the examined HPV types. No cases were positive for HPV-16 or 18. Furthermore, we isolated the recombinant HPV-11 DNA clone from a genomic library constructed with the DNA of RRP tissue. The restriction map of the cloned HPV DNA was identical with the map of known HPV-11 DNA. These results suggest at least that no specific HPV type or subtype is likely to be associated with RRP in Thailand.
复发性呼吸道乳头状瘤病（RRP）在泰国非常普遍。在本研究中，我们通过斑点印迹分析和/或聚合酶链反应检测了25例泰国RRP患者中人类乳头瘤病毒（HPV）DNA的存在情况。分别有84%（21/25）的病例和4%（1/25）的病例HPV-11 DNA和HPV-6 DNA呈阳性。3例（3/25）所有检测的HPV类型均为阴性。没有病例HPV-16或18呈阳性。此外，我们从用RRP组织DNA构建的基因组文库中分离出重组HPV-11 DNA克隆。克隆的HPV DNA的限制性图谱与已知HPV-11 DNA的图谱相同。这些结果至少表明，在泰国，没有特定的HPV类型或亚型可能与RRP相关。
Zotero 插件