Jansman A J, Frohlich A A, Marquardt R R
TNO Nutrition and Food Research, Department of Animal Nutriton and Physiology, Wageningen, The Netherlands.
J Nutr. 1994 Feb;124(2):249-58. doi: 10.1093/jn/124.2.249.
Feeding a casein-based diet with either 400 g/kg of tannin-containing faba bean hulls (Vicia faba L.) (1.41% condensed tannins) or 60 g/kg of a tannin-rich hull extract of faba beans (1.99% condensed tannins) to rats over a period of 7 d resulted in a 2.6 and 1.5 fold increase in weight of the parotid glands, respectively, (P < 0.05) and a corresponding 5.5 and 3.7 fold increase in the level of proline-rich proteins in the glands (P < 0.05). In a dose-response experiment, increasing the level of tannin-rich hull extract in the diet (0.0, 3.8, 7.5, 15.0, 30.0 and 60.0 g/kg) resulted in a linear increase in both the relative size of parotid glands (R2 = 0.90; P < 0.05) and the quantity of proline-rich proteins in the glands (R2 = 0.89; P < 0.05). The apparent digestibility of total (R2 = 0.97) and individual amino acids (R2 varied from 0.27 to 0.99) decreased linearly (P < 0.05). The quantity of proline-rich proteins in the cecum of rats was estimated from the decrease in digestibility of proline, glycine and glutamic acid. The estimated secretions of proline-rich proteins, when calculated on the basis of the three respective amino acids, were 3.5, 3.5 and 3.9 mg of proline-rich proteins per 10 mg of additional hull extract (21.8% condensed tannins). The results indicate that tannins from faba beans stimulate the parotid glands to increase the secretion of proline-rich proteins in rats. The proline-rich proteins then interact with dietary condensed tannins to reduce their antinutritional effects.
给大鼠喂食含400克/千克含单宁蚕豆皮（蚕豆属）（1.41%缩合单宁）或60克/千克富含单宁的蚕豆皮提取物（1.99%缩合单宁）的酪蛋白基日粮，持续7天，结果腮腺重量分别增加了2.6倍和1.5倍（P<0.05），腺体中富含脯氨酸蛋白水平相应增加了5.5倍和3.7倍（P<0.05）。在剂量反应实验中，增加日粮中富含单宁的皮提取物水平（0.0、3.8、7.5、15.0、30.0和60.0克/千克）导致腮腺相对大小（R2 = 0.90；P<0.05）和腺体中富含脯氨酸蛋白数量（R2 = 0.89；P<0.05）呈线性增加。总氨基酸（R2 = 0.97）和单个氨基酸（R2从0.27到0.99不等）的表观消化率呈线性下降（P<0.05）。根据脯氨酸、甘氨酸和谷氨酸消化率的下降来估计大鼠盲肠中富含脯氨酸蛋白的数量。基于三种相应氨基酸计算，每10毫克额外的皮提取物（21.8%缩合单宁）中富含脯氨酸蛋白的估计分泌量分别为3.5、3.5和3.9毫克。结果表明，蚕豆中的单宁刺激大鼠腮腺增加富含脯氨酸蛋白的分泌。富含脯氨酸的蛋白随后与日粮中的缩合单宁相互作用，以降低其抗营养作用。
