Kurlenda J, Byczyńska B, Samet A, Lammek J
Zakład Bakteriologii Klinicznej, PSK nr 1, Gdańsk.
Med Dosw Mikrobiol. 1993;45(2):167-71.
The study was aimed at evaluation of utility of plasmids as epidemiological markers for interspecific differentiation of 200 strains of Klebsiella isolated from patients with generalized infections and hospitalized at intensive care ward of the Gdańsk Medical Academy. Strains isolated from the ward environment were also investigated. In the first phase of this study classical methods of typing were used, such as determination of the biochemical, bacteriocin (susceptibility to 8 bacteriocins) and phage types, and MIC for 9 antibiotics. Basing on these methods it was found that 41 types of Klebsiella pneumoniae, 13 types of Klebsiella oxytoca and 1 type of Klebsiella ozenae occur. Six groups of bacteria were differentiated in the group which had the same type. These strains were investigated for plasmid and restrictive patterns. Identity of types occurring in individual groups was detected. For one of them, a restrictive pattern was determined by cleavage of plasmid DNA by a restrictive Eco RV enzyme. In remaining groups such pattern was not found, both after treatment with Eco RV and Hha I enzyme and this may be associated with modification of the plasmid DNA. Strains belonging to one of these groups did not contain plasmids in their cells.
该研究旨在评估质粒作为流行病学标志物在区分200株从患有全身性感染且在格但斯克医科大学重症监护病房住院的患者中分离出的克雷伯菌种间差异方面的效用。还对从病房环境中分离出的菌株进行了研究。在本研究的第一阶段，使用了经典的分型方法，如生化测定、细菌素（对8种细菌素的敏感性）和噬菌体分型，以及9种抗生素的最低抑菌浓度（MIC）。基于这些方法，发现存在41种肺炎克雷伯菌、13种产酸克雷伯菌和1种奥泽纳克雷伯菌。在具有相同类型的组中区分出了六组细菌。对这些菌株进行了质粒和限制性图谱研究。检测到各个组中出现的类型的一致性。对于其中一组，通过限制性内切酶Eco RV切割质粒DNA确定了限制性图谱。在用Eco RV和Hha I酶处理后，在其余组中未发现这种图谱，这可能与质粒DNA的修饰有关。属于这些组之一的菌株在其细胞中不含有质粒。
