Winter J, Walpole C S, Bevan S, James I F
Sandoz Institute for Medical Research, London, U.K.
Neuroscience. 1993 Dec;57(3):747-57. doi: 10.1016/0306-4522(93)90021-7.
Binding of [3H]resiniferatoxin was seen by autoradiography in sections of rat dorsal root ganglia and the superficial dorsal horn of the spinal cord. Membranes from rat dorsal root ganglia and spinal cord, but not other tissues, had saturable high-affinity binding sites for [3H]resiniferatoxin. A series of capsaicin analogues competed for these sites. The sites probably correspond to capsaicin receptors. Systemic pretreatment of rats with capsaicin caused loss of capsaicin sensitivity in sensory neurons and a reduction in binding of resiniferatoxin to rat dorsal root ganglia, measured by binding assays and autoradiography. Adult rat dorsal root ganglion neurons cultured without nerve growth factor also lost their capsaicin-sensitivity and showed reduced resiniferatoxin binding. Therefore, capsaicin responses in sensory neurons may be regulated by nerve growth factor through control of the number of capsaicin receptors.
通过放射自显影法在大鼠背根神经节切片和脊髓背角浅层观察到了[3H]树脂毒素的结合。大鼠背根神经节和脊髓的膜，而非其他组织，具有可饱和的高亲和力[3H]树脂毒素结合位点。一系列辣椒素类似物竞争这些位点。这些位点可能对应于辣椒素受体。用辣椒素对大鼠进行全身预处理会导致感觉神经元中辣椒素敏感性丧失，并且通过结合测定和放射自显影法测量发现，树脂毒素与大鼠背根神经节的结合减少。在无神经生长因子的情况下培养的成年大鼠背根神经节神经元也丧失了它们的辣椒素敏感性，并表现出树脂毒素结合减少。因此，感觉神经元中的辣椒素反应可能受神经生长因子通过控制辣椒素受体数量来调节。
