细胞内三磷酸腺苷（ATP）改变牛蛙初级传入神经元中延迟整流钾电流的电压依赖性。

Intracellular ATP changes the voltage-dependence of delayed rectifier potassium current in bullfrog primary afferent neurons.

作者信息

Tokimasa T, Tsurusaki M, Ishimatsu M, Akasu T

机构信息

Department of Physiology, Kurume University School of Medicine, Japan.

出版信息

Neurosci Lett. 1993 Dec 12;163(2):138-40. doi: 10.1016/0304-3940(93)90365-r.

DOI:10.1016/0304-3940(93)90365-r

PMID:8309619

Abstract

Dissociated bullfrog dorsal root ganglion cells were voltage-clamped in the whole-cell configuration to study the steady-state activation and inactivation curves for a delayed rectifier potassium current. The 50%-activation of the current occurred at +15 mV when measured with ATP (5 mM) in the pipette solution as opposed to -11 mV with 5'-adenylylimidodiphosphate (AMP-PNP, 5 mM) and -15 mV with adenosine 5'-O-(3-thiotriphosphate) (5 mM). The 50%-inactivation of the current occurred at -6 mV with ATP but at -31 mM with AMP-PNP. The results suggest that intracellular ATP modulates voltage-dependence of the delayed rectifier in amphibian afferent neurons.

摘要

将牛蛙背根神经节细胞分离出来，采用全细胞模式进行电压钳制，以研究延迟整流钾电流的稳态激活和失活曲线。当移液管溶液中含有ATP（5 mM）时，电流的50%激活发生在+15 mV，而含有5'-腺苷酰亚胺二磷酸（AMP-PNP，5 mM）时为-11 mV，含有腺苷5'-O-（3-硫代三磷酸）（5 mM）时为-15 mV。电流的50%失活在含有ATP时发生在-6 mV，但在含有AMP-PNP时为-31 mV。结果表明，细胞内ATP调节两栖类传入神经元中延迟整流器的电压依赖性。