奎宁对牛蛙交感神经元延迟整流型钾电流稳态失活曲线的超极化偏移作用。

Hyperpolarizing shift by quinine in the steady-state inactivation curve of delayed rectifier-type potassium current in bullfrog sympathetic neurons.

作者信息

Kotani S, Hasegawa J, Meng H, Suzuki T, Sato K, Sakakibara M, Takiguchi M, Tokimasa T

机构信息

Department of Physiology, Tokai University School of Medicine, Bohseidai, Isehara, Japan.

出版信息

Neurosci Lett. 2001 Mar 9;300(2):87-90. doi: 10.1016/s0304-3940(01)01554-3.

DOI:10.1016/s0304-3940(01)01554-3

PMID:11207381

Abstract

Whole-cell recordings were made from dissociated bullfrog sympathetic neurons to examine the actions of quinine (1-100 microM) on the steady-state activation and inactivation curves of a delayed rectifier-type potassium current (I(K)). Quinine (EC50 approximately 8 microM) caused a hyperpolarizing shift (approximately 31 mV with 30 microM) in the inactivation curve of I(K) without significantly affecting its activation curve. Quinine (20 microM) was without effects on the voltage-dependence of a rapidly-inactivating A-type potassium current (I(A)). It is concluded that quinine can selectively modulate the voltage-dependence of I(K) in amphibian autonomic neurons.

摘要

通过对分离的牛蛙交感神经元进行全细胞记录，以研究奎宁（1 - 100微摩尔）对延迟整流型钾电流（I(K)）的稳态激活和失活曲线的作用。奎宁（半数有效浓度约为8微摩尔）使I(K)的失活曲线发生超极化偏移（30微摩尔时约为31毫伏），而对其激活曲线无显著影响。奎宁（20微摩尔）对快速失活的A型钾电流（I(A)）的电压依赖性无影响。结论是奎宁可选择性调节两栖类自主神经元中I(K)的电压依赖性。