Forattini O P, Kakitani I, Massad E, Gomes A de C
Department of Epidemiology, School of Public Health, University of S. Paulo, Brazil.
Rev Saude Publica. 1993 Feb;27(1):1-8. doi: 10.1590/s0034-89101993000100001.
Populations of Anopheles (Kerteszia) were sampled fortnightly over a one-year period (August 1991 to July 1992) at Ribeira Valley, S. Paulo State, Brazil. Indoor and outdoor collections were made on human bait at evening crepuscular period. The Polovodova technique for age grading was applied to 3,501 females of Anopheles cruzii and to 416 females of An. bellator. That sample represented 34.4% of the total number of mosquitoes collected. The most abundant species found was An. cruzii. However, An. bellator showed an endophagy that was almost three times greater than that of An. cruzii. The overall parous rate was 25.4% and uniparity was practically dominant one. A proportion of 26.9% of An. cruzii and 12.0% of An. bellator were found to be uniparous. Only three outdoor females of the former species (0.1%) showed biparity. Parity of An. cruzii was higher in females caught indoors than in those caught outdoors. Nevertheless, 497 nulliparous females examined (417 cruzii and 80 bellator) had ovaries that had advanced to Christophers and Mer stages III to V. These results imply that these females had already practised hematophagy. Relating these results to those from the parous females, a high statistical significance was found, leading to the conclusion that gonothophic discordance is a common pattern among these anophelines. Further, these results obtained with human bait catches strongly suggest that nearly 38.0% of these host-seeking females had already taken at least one previous blood-meal. So it is possible that enough time could thus be available for the plasmodian development in the vectors.
1991年8月至1992年7月的一年时间里，每两周在巴西圣保罗州里贝拉河谷对按蚊（克尔泰齐亚按蚊亚属）种群进行一次采样。在傍晚黄昏时段，以人作为诱饵进行室内和室外采集。采用波洛沃多娃年龄分级技术，对3501只克鲁兹按蚊雌蚊和416只贝拉托按蚊雌蚊进行了分析。该样本占所采集蚊子总数的34.4%。发现数量最多的物种是克鲁兹按蚊。然而，贝拉托按蚊的嗜内性几乎是克鲁兹按蚊的三倍。总体产蚊率为25.4%，单配性实际上占主导地位。发现26.9%的克鲁兹按蚊和12.0%的贝拉托按蚊为单配性。前一种按蚊只有三只室外雌蚊（0.1%）表现为双配性。克鲁兹按蚊在室内捕获的雌蚊中的产蚊率高于室外捕获的雌蚊。尽管如此，对497只未产蚊雌蚊（417只克鲁兹按蚊和80只贝拉托按蚊）进行检查时发现，它们的卵巢已发育到克里斯托弗斯和梅尔III至V期。这些结果表明，这些雌蚊已经吸食过血液。将这些结果与产蚊雌蚊的结果相关联时，发现具有高度统计学意义，从而得出结论，性腺发育不一致是这些按蚊中的常见模式。此外，这些用人诱饵捕获的结果强烈表明，这些寻找宿主的雌蚊中近38.0%已经至少吸食过一次血餐。因此，疟原虫在媒介中可能有足够的时间发育。
