Horrocks B R, Schmidtke D, Heller A, Bard A J
Department of Chemistry and Biochemistry, University of Texas at Austin 78712.
Anal Chem. 1993 Dec 15;65(24):3605-14. doi: 10.1021/ac00072a013.
Hydrogen peroxide sensing microelectrodes for scanning electrochemical microscopy (SECM) have been developed and used to measure hydrogen peroxide in the diffusion layer during electrochemical reduction of oxygen on gold and carbon electrodes. These microelectrode biosensors were also used to detect immobilized glucose oxidase through the production of hydrogen peroxide during enzymatic oxidation of glucose. Images of hydrogen peroxide concentration profiles near a platinum microdisk during catalytic decomposition of peroxide and in the diffusion layer of a carbon-platinum composite electrode during oxygen reduction are presented. The factors limiting the spatial resolution (tens of micrometers) and potential applications of the technique are discussed.
已开发出用于扫描电化学显微镜（SECM）的过氧化氢传感微电极，并用于测量在金电极和碳电极上进行氧电化学还原过程中扩散层中的过氧化氢。这些微电极生物传感器还用于通过葡萄糖酶促氧化过程中过氧化氢的产生来检测固定化的葡萄糖氧化酶。展示了在过氧化物催化分解过程中铂微盘附近以及在氧还原过程中碳 - 铂复合电极扩散层中过氧化氢浓度分布的图像。讨论了限制该技术空间分辨率（几十微米）的因素及其潜在应用。
