Kalina S L, Mottram P L
Microsurgery Research Unit, St Vincent's Hospital, Victoria, Australia.
Aust N Z J Surg. 1993 Mar;63(3):213-6. doi: 10.1111/j.1445-2197.1993.tb00521.x.
A modified technique for the transplantation of primarily vascularized mouse kidneys is described in which the donor was prepared by exposure of the left kidney, dissection of the suprarenal aorta and vena cava and of the ureter and bladder. The recipient was prepared by exposure of the infrarenal aorta and vena cava and isolation of approximately 1 cm of these vessels by proximal and distal 4/0 silk ties. The donor kidney was then perfused and removed to the recipient with the ureter and bladder. The transplant was completed by microsurgical end-to-side anastomosis of the aorta to aorta and vena cava to vena cava and the donor ureter was attached to the recipient bladder via a bladder patch. This technique differed significantly from the previously published technique in using cuffs of donor suprarenal aorta and vena cava rather than dissecting the base of the renal vessels. These changes made the operation easier and reduced ischaemia time from 35-40 to 25-30 min, a substantial improvement which increased both organ and recipient survival.
本文描述了一种改良的主要血管化小鼠肾脏移植技术，其中供体的制备方法是暴露左肾，解剖肾上腺主动脉、腔静脉以及输尿管和膀胱。受体的制备方法是暴露肾下主动脉和腔静脉，并用近端和远端4/0丝线结扎隔离约1厘米长的这些血管。然后对供体肾脏进行灌注，并将其与输尿管和膀胱一起移至受体处。通过主动脉与主动脉、腔静脉与腔静脉的显微外科端侧吻合完成移植，供体输尿管通过膀胱补片连接到受体膀胱。该技术与先前发表的技术有显著不同，它使用供体肾上腺主动脉和腔静脉的袖套，而不是解剖肾血管基部。这些改变使手术更容易，并将缺血时间从35 - 40分钟减少到25 - 30分钟，这是一个显著的改进，提高了器官和受体的存活率。
