Kitamura K, Besansky N J, Rudolph D, Nutman T B, Folks T M, Lal R B
Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
AIDS Res Hum Retroviruses. 1993 Nov;9(11):1167-72. doi: 10.1089/aid.1993.9.1167.
Accumulation of unintegrated human T lymphotropic virus (HTLV) DNA was analyzed in long-term T cell lines infected with HTLV type I (HTLV-I) or type II (HTLV-II). By using a polymerase chain reaction-based assay, amplified products of expected size were obtained in all of the HTLV-I-infected (n = 7) and HTLV-II-infected (n = 8) cell lines. The signal intensities of the hybridizing band varied greatly among the cell lines and did not correlate with HTLV p24gag antigen production. Further analysis of HTLV-I-infected clones demonstrated considerable variability in the unintegrated DNA accumulation, suggesting that either the epigenetic status of the host cell or some environmental factor determines the occurrence of unintegrated DNA. The presence of lower levels of unintegrated DNA in most of the HTLV-infected, long-term cell lines presumably results in persistent noncytopathic infection.
在感染了I型人类嗜T淋巴细胞病毒（HTLV-I）或II型人类嗜T淋巴细胞病毒（HTLV-II）的长期T细胞系中，分析了未整合的HTLV DNA的积累情况。通过基于聚合酶链反应的检测方法，在所有感染HTLV-I（n = 7）和感染HTLV-II（n = 8）的细胞系中均获得了预期大小的扩增产物。杂交带的信号强度在各细胞系之间差异很大，且与HTLV p24gag抗原产生无关。对感染HTLV-I的克隆进行的进一步分析表明，未整合DNA的积累存在相当大的变异性，这表明宿主细胞的表观遗传状态或某些环境因素决定了未整合DNA的出现。在大多数感染HTLV的长期细胞系中，未整合DNA水平较低，这可能导致持续性的无细胞病变感染。
