一种用于在24小时内检测食品中沙门氏菌的新型半自动筛查方法的评估。

Evaluation of a new semi-automated screening method for the detection of Salmonella in foods within 24 h.

作者信息

Krusell L, Skovgaard N

机构信息

Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Frederiksberg, Denmark.

出版信息

Int J Food Microbiol. 1993 Nov 26;20(3):123-30. doi: 10.1016/0168-1605(93)90105-p.

DOI:10.1016/0168-1605(93)90105-p

PMID:8312139

Abstract

A new method for the detection of Salmonella in foods and feeds, 'The EiaFoss Salmonella Method' has been performance tested in comparison with a traditional technique, the Rappaport-Vassiliadis cultural procedure, as reference method. The new method is based on a two step enrichment procedure (19 and 3 h) using two different pre-enrichment broths (Salmonella enrichment broth, SEB I and SEB II) for raw and processed foods, followed by an automated enzyme immunoassay (EIA) carried out in the 'EiaFoss analyser'. A total of 161 food and nine feed samples were investigated. Only naturally contaminated samples were used. Viable Salmonella were recovered in 30 samples using either method. When using the EiaFoss Salmonella Method 13% more samples (27:24) were proved salmonella-positive compared with the reference method. The explanation for the better performance of the EiaFoss Salmonella Method is discussed and attributed in part to better enrichment procedures when using SEB I or SEB II.

摘要

一种用于检测食品和饲料中沙门氏菌的新方法——“EiaFoss沙门氏菌检测法”，已与传统技术拉帕波特 - 瓦西里亚迪斯培养法作为参考方法进行了性能测试。新方法基于两步富集程序（19小时和3小时），针对生食品和加工食品使用两种不同的预富集肉汤（沙门氏菌富集肉汤，SEB I和SEB II），随后在“EiaFoss分析仪”中进行自动化酶免疫分析（EIA）。总共对161份食品和9份饲料样品进行了调查。仅使用自然污染的样品。两种方法在30个样品中均检测到了活的沙门氏菌。与参考方法相比，使用EiaFoss沙门氏菌检测法时，沙门氏菌阳性样品多了13%（27:24）。本文讨论了EiaFoss沙门氏菌检测法表现更优的原因，部分原因是使用SEB I或SEB II时富集程序更佳。