小鼠组织蛋白酶B mRNA的多个前导序列？

Multiple leader sequences for mouse cathepsin B mRNA?

作者信息

Rhaissi H, Béchet D, Ferrara M

机构信息

Unité Expression des Protéases Lysosomales, INRA Theix, Saint-Genès-Champanelle, France.

出版信息

Biochimie. 1993;75(10):899-904. doi: 10.1016/0300-9084(93)90046-u.

DOI:10.1016/0300-9084(93)90046-u

PMID:8312393

Abstract

We previously described the gene structure of murine cathepsin B. Our results suggested that the 5'-untranslated region (leader) is interrupted by a large intron. The second exon (exon-2) contains the translation initiation site. To characterize the leader region, a rapid amplification of cDNA ends (RACE) procedure was developed. The PCR products were directly cloned and sequenced. Nucleotide sequence analyses revealed three different 5'-cDNA ends, suggesting the existence of three different leader regions. In addition to the leader (LA) previously characterized, we now describe two other 5'-untranslated regions, LB and LC. Leader LB is located 2.3 kb upstream exon-2, and leader LC corresponds to the 3'-end of the first intron and is thus contiguous to exon-2. Our results suggest for murine cathepsin B gene the presence of multiple promoters, and possibly the expression of multiple mRNAs differing in their leader region.

摘要

我们之前描述了小鼠组织蛋白酶B的基因结构。我们的结果表明，5'-非翻译区（前导序列）被一个大的内含子打断。第二个外显子（外显子2）包含翻译起始位点。为了表征前导序列区域，我们开发了一种cDNA末端快速扩增（RACE）方法。PCR产物直接克隆并测序。核苷酸序列分析揭示了三个不同的5'-cDNA末端，表明存在三个不同的前导序列区域。除了之前表征的前导序列LA，我们现在描述另外两个5'-非翻译区，LB和LC。前导序列LB位于外显子2上游2.3 kb处，前导序列LC对应于第一个内含子的3'-末端，因此与外显子2相邻。我们的结果表明，小鼠组织蛋白酶B基因存在多个启动子，并且可能表达前导序列区域不同的多种mRNA。