Stachecki J J, Yelian F D, Schultz J F, Leach R E, Armant D R
C. S. Mott Center for Human Growth and Development, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Biol Reprod. 1994 Jan;50(1):1-9. doi: 10.1095/biolreprod50.1.1.
To extend our previous finding that ethanol stimulates murine preimplantation development, we focused in the current study on the cavitation and expansion of the blastocyst. Ethanol stimulation of blastocyst cavitation and expansion was optimal at a concentration of 0.1% and required only a 5-min exposure. Because intracellular levels of calcium were transiently increased in the ethanol-exposed embryos, we determined the effect of directly increasing calcium on the rates of blastocyst cavitation and expansion. Treatment with the calcium ionophore, A23187, altered development much as did ethanol, indicating that ethanol may stimulate preimplantation development by increasing the intracellular calcium concentration. The relationship between intracellular calcium levels and blastocoel volume suggests that morphogenetic events during preimplantation development, particularly cavitation and blastocyst formation, may be regulated through modulation of intracellular calcium levels.
为了扩展我们之前的发现，即乙醇可刺激小鼠植入前胚胎发育，我们在当前研究中聚焦于囊胚的空化和扩张。乙醇刺激囊胚空化和扩张的最佳浓度为0.1%，且仅需暴露5分钟。由于乙醇处理的胚胎细胞内钙水平短暂升高，我们确定了直接增加钙对囊胚空化和扩张速率的影响。用钙离子载体A23187处理后，胚胎发育的改变与乙醇处理时相似，这表明乙醇可能通过增加细胞内钙浓度来刺激植入前胚胎发育。细胞内钙水平与囊胚腔体积之间的关系表明，植入前胚胎发育过程中的形态发生事件，特别是空化和囊胚形成，可能通过调节细胞内钙水平来调控。
