通过固相酶联免疫吸附测定法检测免疫反应性裂褶多糖

Detection of immunoreactive schizophyllan by solid-phase enzyme-linked immunosorbent assay.

作者信息

Hirata A, Itoh W, Komoda M, Tabata K, Itoyama S, Sugawara I

机构信息

Research Laboratory, Taito Co., Ltd., Kobe, Japan.

出版信息

Biol Pharm Bull. 1993 Nov;16(11):1091-3. doi: 10.1248/bpb.16.1091.

DOI:10.1248/bpb.16.1091

PMID:8312861

Abstract

In order to detect immunoreactive schizophyllan (SPG), one of the beta-1,3-glucans in solution, a murine anti-schizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA), and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 microgram/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood.

摘要

为了检测溶液中的β-1,3-葡聚糖之一——免疫反应性裂褶菌多糖（SPG），一种抗裂褶菌多糖鼠单克隆抗体SPG1-HS被用作固相酶联免疫吸附测定（ELISA）的一抗。制备了多糖-牛血清白蛋白（BSA）和多糖-牛血红蛋白（Hb）偶联物作为抗原。ELISA中490nm处的吸光度与SPG-BSA或SPG-Hb偶联物中的SPG浓度成正比，使得能够准确测量浓度超过1.0微克/毫升的SPG。此外，该ELISA以类似方式检测人血清中SPG-BSA和SPG-Hb偶联物中的SPG。这些结果表明该ELISA适用于测定人体组织和血液中SPG-BSA和SPG-Hb偶联物中的SPG浓度。