Sayre M H, Kornberg R D
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
Cell Mol Biol Res. 1993;39(4):349-54.
Fractionation of yeast whole-cell extract has led to the identification and purification of four novel proteins required for promoter-dependent transcription by RNA polymerase II in vitro. These initiation factors have been shown to be related in structure and function to RNA polymerase II initiation factors purified from mammalian cells. When combined with the well known TATA-binding protein, the entire set of initiation factors proves necessary and sufficient for accurate initiation from a variety of eukaryotic promoters. Studies with this defined transcription system have revealed the existence of additional proteins required for transcriptional activation or derepression by enhancer-binding regulatory proteins.
对酵母全细胞提取物进行分级分离，已鉴定并纯化出四种新型蛋白质，它们是RNA聚合酶II在体外进行启动子依赖性转录所必需的。这些起始因子在结构和功能上已被证明与从哺乳动物细胞中纯化出的RNA聚合酶II起始因子相关。当与众所周知的TATA结合蛋白结合时，整套起始因子被证明对于从多种真核启动子进行准确起始是必要且充分的。对这个明确的转录系统的研究揭示了增强子结合调节蛋白进行转录激活或去抑制所需的其他蛋白质的存在。
