Kameda Y, Kameya T, Frankfurter A
Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
Brain Res. 1993 Nov 19;628(1-2):121-7. doi: 10.1016/0006-8993(93)90946-k.
The localization of a neuron-specific beta-tubulin isotype in the ultimobranchial glands from chickens at various stages of development was studied by means of light- and electron microscopic immunohistochemistry with a monoclonal antibody (TuJ1) against the beta-tubulin isotype, c beta 4. At 8 days of incubation, many C cells in the ultimobranchial glands showed immunoreactivity for TuJ1 invariable degrees, weak to intense. At 12 days of incubation, a vast majority of C cells were intensely immunoreactive for TuJ1, and further TuJ1-immunoreactive nerve fibers were distributed in the ultimobranchial glands. At 14 and 16 days of incubation, intense immunoreactivity for TuJ1 was sustained in the C cells. Electron microscopic analyses revealed that TuJ1 immunoreactivity was diffusely distributed throughout the cytoplasm and also localized on the secretory granules of C cells at these stages. TuJ1 immunoreactivity in the C cells started to decrease at late stages of embryonic development. At the hatching period, dense distributions of TuJ1-immunoreactive nerve fibers were observed in the ultimobranchial glands, whereas TuJ1 immunoreactivity of the C cells became very weak. In 10-day-old chickens, TuJ1 immunoreactivity was restricted to the nerve fibers.
利用针对β-微管蛋白同型异构体cβ4的单克隆抗体（TuJ1），通过光镜和电镜免疫组织化学方法，研究了鸡不同发育阶段后鳃腺中神经元特异性β-微管蛋白同型异构体的定位。在孵化第8天，后鳃腺中的许多C细胞对TuJ1呈现出不同程度的免疫反应性，从弱到强。在孵化第12天，绝大多数C细胞对TuJ1呈现强烈免疫反应性，并且在鳃后腺中有更多TuJ1免疫反应性神经纤维分布。在孵化第14天和16天，C细胞中对TuJ1的强烈免疫反应性持续存在。电镜分析显示，在这些阶段，TuJ1免疫反应性在整个细胞质中呈弥漫性分布，并且也定位于C细胞的分泌颗粒上。C细胞中的TuJ1免疫反应性在胚胎发育后期开始下降。在孵化期，在后鳃腺中观察到TuJ1免疫反应性神经纤维密集分布，而C细胞的TuJ1免疫反应性变得非常弱。在10日龄鸡中，TuJ1免疫反应性局限于神经纤维。
