Philpott D J, Kirk D R, Butzner J D
Gastrointestinal Research Group, University of Calgary, Canada.
Can J Physiol Pharmacol. 1993 Sep;71(9):650-6. doi: 10.1139/y93-095.
We examined the role of luminal versus systemic factors in promoting intestinal recovery during the refeeding of previously malnourished infant rabbits. Malnutrition was induced by litter expansion at 7 days of age. A 20-cm Thirty-Vella (T-V) loop was created in the intestine of each malnourished and dietary control animal at 21 days of age. Beginning on day 28, controls and one half of the malnourished group (malnourished-refed) were fed chow ad libitum, whereas the remainder of the malnourished group received half the amount of chow given to the malnourished-refed group. On day 35, proximal and distal segments from the intact intestine that remained in continuity as well as segments from the excluded T-V loops were examined. Malnutrition severely reduced mucosal mass and disaccharidase activities in the intact distal intestine. A brief period of refeeding led to a rapid recovery of these parameters. In contrast, the excluded T-V loop segments of the control, malnourished, and malnourished-refed groups all displayed decreased mucosal mass and impaired function to a degree similar to that observed in the intact distal segment from the malnourished group. These results indicate that luminal factors are essential for (i) the maintenance of normal intestinal structure and function in infant rabbits and (ii) the promotion of mucosal repair following nutritional rehabilitation of malnourished animals.
我们研究了管腔因素与全身因素在促进先前营养不良的幼兔重新喂养期间肠道恢复中的作用。在7日龄时通过增加同窝幼崽数量诱导营养不良。在21日龄时，在每只营养不良和饮食对照动物的肠道中创建一个20厘米长的Thirty-Vella（T-V）肠袢。从第28天开始，对照组和一半营养不良组（重新喂养的营养不良组）自由采食饲料，而其余营养不良组接受的饲料量是重新喂养的营养不良组的一半。在第35天，检查完整且保持连续的肠道的近端和远端节段以及排除的T-V肠袢的节段。营养不良严重降低了完整远端肠道的粘膜质量和双糖酶活性。短暂的重新喂养导致这些参数迅速恢复。相比之下，对照组、营养不良组和重新喂养的营养不良组的排除的T-V肠袢节段均显示粘膜质量下降和功能受损，其程度与营养不良组完整远端节段中观察到的相似。这些结果表明，管腔因素对于（i）维持幼兔正常肠道结构和功能以及（ii）促进营养不良动物营养康复后的粘膜修复至关重要。
