Kakutani M, Murakami M, Okamoto H, Kudo I, Inoue K
Japan Tobacco Inc., Pharmaceutical Research Laboratories, Osaka, Japan.
FEBS Lett. 1994 Feb 14;339(1-2):76-8. doi: 10.1016/0014-5793(94)80388-9.
In order to investigate the role of type II phospholipase A2 (PLA2) in the inflammatory process, the effect of a monoclonal antibody specific to type II PLA2 on carrageenan-induced pleurisy was studied in rats. Intravenous injection of the antibody (MB5.2), which inhibits the catalytic activity of type II PLA2, significantly reduced both the pleural exudate volume and the intrapleural leukocyte count, while a control antibody did not have any appreciable effect. MB5.2 caused no change in the level of type II PLA2 in the pleural fluid. These results suggest that type II PLA2 generated at inflamed sites, at least in part, have a crucial role in the pathogenesis of acute inflammation.
为了研究II型磷脂酶A2（PLA2）在炎症过程中的作用，在大鼠中研究了针对II型PLA2的单克隆抗体对角叉菜胶诱导的胸膜炎的影响。静脉注射抑制II型PLA2催化活性的抗体（MB5.2）可显著降低胸腔渗出液体积和胸腔内白细胞计数，而对照抗体则没有任何明显作用。MB5.2对胸腔积液中II型PLA2的水平没有影响。这些结果表明，在炎症部位产生的II型PLA2至少部分地在急性炎症的发病机制中起关键作用。
