Newgard C B
Gillford Laboratories for Diabetes Research, University of Texas Southwestern Medical Center, Dallas 75235.
Diabetes. 1994 Mar;43(3):341-50. doi: 10.2337/diab.43.3.341.
In diabetes, insulin secretion is either completely absent (insulin-dependent diabetes mellitus [IDDM]) or inappropriately regulated (non-insulin-dependent diabetes mellitus [NIDDM]). In recent years, new insights into the molecular and biochemical mechanism(s) of fuel-mediated insulin release coupled with advances in gene transfer technology have led to the investigation of molecular strategies for replacement of normal insulin delivery function. Such initiatives have included attempts to engineer glucose-stimulated insulin secretion in cell lines that might serve as surrogates for islets in IDDM. The development of DNA virus gene transfer systems of remarkable efficiency also has suggested ways in which the beta-cell dysfunction of NIDDM might ultimately be repaired by gene therapy. The emerging work in these areas and implications for the future are summarized in this perspective.
在糖尿病中，胰岛素分泌要么完全缺失（胰岛素依赖型糖尿病[IDDM]），要么调节不当（非胰岛素依赖型糖尿病[NIDDM]）。近年来，对燃料介导的胰岛素释放的分子和生化机制的新见解，再加上基因转移技术的进步，引发了对替代正常胰岛素递送功能的分子策略的研究。这些举措包括尝试在细胞系中设计葡萄糖刺激的胰岛素分泌，这些细胞系可作为IDDM中胰岛的替代物。高效DNA病毒基因转移系统的发展也提出了通过基因治疗最终修复NIDDM中β细胞功能障碍的方法。本文将概述这些领域的新进展及其对未来的影响。
