Tobi S E, Neary D, Itzhaki R F
Department of Optometry and Vision Sciences, UMIST, Manchester, UK.
Gerontology. 1993;39(5):241-51. doi: 10.1159/000213539.
We have previously shown that lymphocytes from Alzheimer's disease (AD) patients are hypersensitive to ionising radiation, as revealed by their higher levels of dicentrics than those from age-matched, normal individuals. We have now examined the sensitivity of these cells to N-methyl-N-nitrosourea or methyl methanesulphonate, using a variety of assays, namely unscheduled DNA synthesis, viability, sister chromatid exchange and, via high performance liquid chromatography, level of methylation and repair of the adducts, 7-methylguanine, 0(6)-methylguanine and 3-methyladenine. We have found that AD lymphocytes and age-matched normal lymphocytes respond similarly to methylation damage, as assessed by all these techniques.
我们之前已经表明，阿尔茨海默病（AD）患者的淋巴细胞对电离辐射高度敏感，这表现为与年龄匹配的正常个体相比，其双着丝粒水平更高。我们现在使用多种检测方法，即非程序性DNA合成、活力、姐妹染色单体交换，以及通过高效液相色谱法检测加合物、7-甲基鸟嘌呤、O(6)-甲基鸟嘌呤和3-甲基腺嘌呤的甲基化水平和修复情况，来研究这些细胞对N-甲基-N-亚硝基脲或甲磺酸甲酯的敏感性。我们发现，通过所有这些技术评估，AD淋巴细胞和年龄匹配的正常淋巴细胞对甲基化损伤的反应相似。
