Boerrigter M E, Vijg J
Division on Aging, Harvard Medical School, Boston, MA 02215.
Age Ageing. 1993 Jan;22(1):S44-52. doi: 10.1093/ageing/22.suppl_1.s44.
Using the alkaline filter elution technique we determined the induction and disappearance of single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer disease (AD) patients and from 48 healthy, age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU), methyl methanesulphonate (MMS), or gamma (gamma)-radiation. No differences in SSB disappearance between AD patients and controls were observed after treatment of PBL with MMS or gamma-rays. After treatment with ENU, however, the amount of SSB disappearance was significantly lower in PBL from familial, but not in PBL from sporadic AD patients. ENU repair in PBL from neurological controls was comparable to that found in normal age-matched controls, indicating that the lower amount of ENU repair in familial AD patients is not a consequence of neuronal degeneration. These tentative findings are discussed in relation to the aetiology of AD.
我们运用碱性滤膜洗脱技术，测定了43例阿尔茨海默病（AD）患者以及48例年龄和性别匹配的健康对照受试者新鲜分离的外周血淋巴细胞（PBL）在体外暴露于N-乙基-N-亚硝基脲（ENU）、甲磺酸甲酯（MMS）或γ射线后单链断裂（SSB）的诱导和消失情况。在用MMS或γ射线处理PBL后，未观察到AD患者与对照之间SSB消失存在差异。然而，在用ENU处理后，家族性AD患者PBL中SSB消失的量显著低于散发性AD患者PBL中的量。神经学对照受试者PBL中的ENU修复与年龄匹配的正常对照者相当，这表明家族性AD患者中ENU修复量较低并非神经元变性的结果。我们结合AD的病因学对这些初步发现进行了讨论。
