Prònai L, Blåzovics A, Horvåth M, Lång I, Fehér J
2nd Department of Medicine, Semmelweis University Medical School, Budapest, Hungary.
Free Radic Res Commun. 1993;19(5):287-96. doi: 10.3109/10715769309056517.
Electron paramagnetic resonance/spin trapping studies were applied, to verify the superoxide radical scavenging activity of two non-toxic, water soluble dihydroquinoline type antioxidants, CH402 (Na-2,2-dimethyl-1,2-dihydroquinoline-4-yl methane sulphonate and MTDQ-DA (6,6-methylene bis 2,2-dimethyl-4-methane sulphonic acid: Na-1,2-dihydroquinoline). Results were compared with other indirect methods such as the amperometric, spectrophotometric and luminometric methods, respectively. Both dihydroquinoline type antioxidants scavenged superoxide in vitro specifically. MTDQ-DA scavenged superoxide an order of magnitude faster than CH-402. Neither CH402 nor MTDQ-DA affected the hypoxanthine/xanthine oxidase superoxide generating system, nor did they inhibit xanthine oxidase directly.
采用电子顺磁共振/自旋捕获研究，以验证两种无毒的水溶性二氢喹啉类抗氧化剂CH402（2,2 - 二甲基 - 1,2 - 二氢喹啉 - 4 - 基甲烷磺酸钠）和MTDQ - DA（6,6 - 亚甲基双2,2 - 二甲基 - 4 - 甲磺酸：1,2 - 二氢喹啉钠）的超氧阴离子清除活性。分别将结果与其他间接方法如安培法、分光光度法和发光法进行比较。两种二氢喹啉类抗氧化剂在体外均能特异性清除超氧阴离子。MTDQ - DA清除超氧阴离子的速度比CH - 402快一个数量级。CH402和MTDQ - DA均不影响次黄嘌呤/黄嘌呤氧化酶超氧阴离子生成系统，也不直接抑制黄嘌呤氧化酶。
