Blázovics A, György I, Zsinka A J, Biacs P, Földiák G, Feher J
Second Department of Medicine, Semmelweis Medical University, Budapest, Hungary.
Free Radic Res Commun. 1989;6(4):217-26. doi: 10.3109/10715768909073474.
The non-toxic and water soluble dihydroquinoline type antioxidants: CH 402 (Na-2,2-dimethyl-1,2-dihydroquinoline-4-yl methane sulphonate) and MTDQ-DA (6,6-methylene bis 2,2-dimethyl-4-methane sulphonic acid: Na-1,2-dihydroquinoline) were studied in various in vitro tests in which oxygen free radicals were generated. Both compounds were shown to scavenge superoxide radical anions O2- produced in aqueous solution by pulse radiolysis with rate constants k (O2- + MTDQ-DA) = 4.10(8) dm3 mol-1 s-1 and k (O2- + CH 402) = 1.5.10(7) dm3 mol-1 s-1. CH 402 and MTDQ-DA reduced the H2O2 produced in the glucose-glucose oxidase reaction, which was detected by the luminol + hemin reaction with a chemiluminometric method. The dihydroquinoline type substrates inhibited the NADPH-induced and Fe3+ - stimulated lipid peroxidation and the ascorbic acid-induced non-enzymatic peroxidation pathways in microsomal fractions of rat and mouse liver.
CH 402（2,2 - 二甲基 - 1,2 - 二氢喹啉 - 4 - 基甲烷磺酸钠）和MTDQ - DA（6,6 - 亚甲基双（2,2 - 二甲基 - 4 - 甲磺酸：1,2 - 二氢喹啉钠））进行了各种体外试验研究，这些试验会产生氧自由基。在脉冲辐解产生水溶液中超氧阴离子自由基O2-的试验中，已证明这两种化合物均能清除该自由基，速率常数分别为k（O2- + MTDQ - DA）= 4.10×10(8) dm3 mol-1 s-1和k（O2- + CH 402）= 1.5×10(7) dm3 mol-1 s-1。CH 402和MTDQ - DA减少了葡萄糖 - 葡萄糖氧化酶反应中产生的H2O2，该反应通过鲁米诺 + 血红素反应采用化学发光法进行检测。二氢喹啉类底物抑制了NADPH诱导和Fe3+刺激的脂质过氧化反应以及抗坏血酸诱导的大鼠和小鼠肝脏微粒体组分中的非酶促过氧化反应途径。
