van Geel I P, Oppelaar H, Oussoren Y G, Stewart F A
Division of Experimental Therapy (H6), The Netherlands Cancer Institute, Amsterdam.
Int J Cancer. 1994 Jan 15;56(2):224-8. doi: 10.1002/ijc.2910560214.
The influence of photodynamic therapy (PDT) on vascular perfusion was investigated in 2 s.c. mouse tumours, a radiation-induced fibrosarcoma (RIF I) and a squamous-cell carcinoma (SCCVII). The 86Rb extraction technique was used to measure changes in perfusion relative to cardiac output at various intervals after interstitial PDT. Control groups showed that vascular perfusion in the RIF I tumours decreased with increasing tumour size. For both tumours, of constant size, vascular perfusion decreased to less than 10% of control values within 5 min after high PDT doses. Significant decreases in vascular perfusion were also seen after lower, sub-curative doses. Thereafter there was slow recovery towards control levels. Photofrin given at shorter intervals before illumination generally resulted in even larger decreases in tumour perfusion, and slower recovery. Comparison of tumour perfusion measurements after PDT with tumour response revealed an inverse correlation with tumour growth delay both for the RIF I and for the SCCVII tumours. PDT with sub-curative light doses appears to decrease vascular perfusion in the RIF I and SCCVII for a period of at least 24 hr. The most severe reductions in tumour blood flow were associated with the longest regrowth delays, indicating a major role of vascular damage in tumour response to PDT.
在两种皮下小鼠肿瘤（一种辐射诱导的纤维肉瘤（RIF I）和一种鳞状细胞癌（SCCVII））中研究了光动力疗法（PDT）对血管灌注的影响。采用⁸⁶Rb提取技术来测量间质PDT后不同时间间隔相对于心输出量的灌注变化。对照组显示，RIF I肿瘤中的血管灌注随着肿瘤大小的增加而降低。对于两种大小恒定的肿瘤，在高剂量PDT后5分钟内，血管灌注降至对照值的10%以下。较低的亚治愈剂量后也观察到血管灌注显著降低。此后，血管灌注缓慢恢复至对照水平。在光照前较短间隔给予光敏剂通常会导致肿瘤灌注的更大降低和更慢的恢复。PDT后肿瘤灌注测量结果与肿瘤反应的比较显示，RIF I和SCCVII肿瘤的肿瘤生长延迟均呈负相关。亚治愈光剂量的PDT似乎在至少24小时内降低了RIF I和SCCVII中的血管灌注。肿瘤血流的最严重减少与最长的再生长延迟相关，表明血管损伤在肿瘤对PDT的反应中起主要作用。
