Pavliak V, Brisson J R, Michon F, Uhrín D, Jennings H J
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
J Biol Chem. 1993 Jul 5;268(19):14146-52.
The L3 immunotype lipopolysaccharide (LPS) of Neisseria meningitidis was subjected to degradation procedures, which produced a number of different oligosaccharide fragments. The high resolution 1H and 13C NMR spectroscopic analyses of these oligosaccharides yielded structural information on a number of different regions of the LPS. For example, from one oligosaccharide, it was found that the endogenous sialylation of the meningococcal LPS occurs at O-3 of the terminal beta-D-galactopyranosyl residue of its lacto-N-neotetraose antenna in the alpha-D-configuration. From another, it was also established that the dominant structural feature responsible for L3 epitope specificity is the presence of a phosphorylethanolamine substituent at O-3 of the penultimate heptopyranosyl residue of its other antenna. In addition from information obtained with another oligosaccharide the structure of the 3-deoxy-D-manno-octulosonic acid disaccharide region of the L3 LPS was also elucidated. From all the above cumulative data plus some published data, it was then possible to reconstruct the complete structure of the entire native L3 LPS.
对脑膜炎奈瑟菌的L3免疫型脂多糖（LPS）进行了降解处理，产生了许多不同的寡糖片段。对这些寡糖进行的高分辨率1H和13C NMR光谱分析，得出了LPS多个不同区域的结构信息。例如，从一种寡糖中发现，脑膜炎奈瑟菌LPS的内源性唾液酸化发生在其乳糖-N-新四糖天线末端β-D-吡喃半乳糖基残基的O-3位，呈α-D-构型。从另一种寡糖中还确定，负责L3表位特异性的主要结构特征是其另一天线倒数第二个庚糖吡喃糖基残基的O-3位存在磷酸乙醇胺取代基。此外，根据从另一种寡糖获得的信息，还阐明了L3 LPS的3-脱氧-D-甘露-辛酮糖酸二糖区域的结构。根据上述所有累积数据以及一些已发表的数据，进而有可能重建整个天然L3 LPS的完整结构。
