Kaijser G P, Beijnen J H, Jeunink E L, Bult A, Keizer H J, de Kraker J, Underberg W J
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
J Chromatogr. 1993 May 5;614(2):253-9. doi: 10.1016/0378-4347(93)80316-v.
A derivatization high-performance liquid chromatographic method with ultraviolet detection to monitor the plasma concentration of chloroacetaldehyde, a neurotoxic metabolite of oxazaphosphorine drugs, is presented. To prevent the rapid degradation of chloroacetaldehyde, the plasma samples are stabilized with formaldehyde. The method is linear in the concentration range 1-250 nmol/ml. Blood samples from a patient who was treated with a ten-day continuous infusion of ifosfamide were assayed. The chloroacetaldehyde concentrations did not exceed 10 nmol/ml.
本文介绍了一种采用紫外检测的衍生化高效液相色谱法，用于监测氮杂磷类药物的神经毒性代谢物氯乙醛的血浆浓度。为防止氯乙醛快速降解，血浆样品用甲醛进行稳定处理。该方法在1-250 nmol/ml的浓度范围内呈线性。对一名接受了十天异环磷酰胺持续输注治疗的患者的血样进行了检测。氯乙醛浓度未超过10 nmol/ml。
