Carlin A S, Simmons J E, el-Arini S K, Shiu G K
Biopharmaceutics Research Branch, Food and Drug Administration, Washington, DC 20204.
J Chromatogr. 1993 May 5;614(2):324-7. doi: 10.1016/0378-4347(93)80325-x.
A rapid gas chromatographic assay has been developed for the separation and determination of khellin in human serum. Using a DB-17 capillary column and a simple chloroform extraction, khellin and an internal standard, trioxsalen, were separated from endogenous substances without further clean-up. Spiked serum samples in the range 0.11-1.1 micrograms/ml were assayed and a linear calibration curve obtained.
已开发出一种快速气相色谱分析法，用于分离和测定人血清中的凯林。使用DB - 17毛细管柱并通过简单的氯仿萃取，无需进一步净化即可将凯林和内标三甲沙林与内源性物质分离。对浓度范围为0.11 - 1.1微克/毫升的加标血清样本进行了分析，并获得了线性校准曲线。
