人卵泡液中高水平的免疫反应性内皮素-1

High levels of immunoreactive endothelin-1 in human follicular fluids.

作者信息

Kamada S, Kubota T, Taguchi M, Aso T

机构信息

Department of Obstetrics and Gynaecology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.

出版信息

Hum Reprod. 1993 May;8(5):674-7. doi: 10.1093/oxfordjournals.humrep.a138118.

DOI:10.1093/oxfordjournals.humrep.a138118

PMID:8314957

Abstract

Follicular fluids were obtained from 180 follicles of 15 women undergoing follicular aspiration for in-vitro fertilization. Follicular development was induced by a combination of buserelin acetate and human menopausal gonadotrophin. Endothelin-1 (ET-1) concentrations in human follicular fluids were determined by specific radioimmunoassay. ET-1-like immunoreactivity (ET-1-LI) ranged from 338 to 928 pg/ml. ET-1-LI concentrations in follicular fluids obtained from immature (< 15 mm) follicles were significantly higher than those from mature (15-25 mm) and post-mature (> or = 25 mm) follicles. No correlation was found between the concentration of ET-1-LI, on the one hand, and that of oestradiol, progesterone, testosterone, prolactin, luteinizing hormone, insulin-like growth factor-I, prostaglandin E2 or platelet activating factor on the other, in follicular fluids. However, a significant positive correlation was observed between ET-1-LI concentration and follicle stimulating hormone and IGF-II concentrations, respectively. These data suggest that the high concentration of ET-1 found in follicular fluids may play some physiological role in follicular development.

摘要

从15名接受体外受精卵泡抽吸术的女性的180个卵泡中获取卵泡液。通过醋酸布舍瑞林和人绝经期促性腺激素联合诱导卵泡发育。采用特异性放射免疫分析法测定人卵泡液中内皮素-1（ET-1）的浓度。ET-1样免疫反应性（ET-1-LI）范围为338至928 pg/ml。从未成熟（<15 mm）卵泡获得的卵泡液中ET-1-LI浓度显著高于成熟（15 - 25 mm）和过成熟（≥25 mm）卵泡。在卵泡液中，一方面ET-1-LI浓度与雌二醇、孕酮、睾酮、催乳素、黄体生成素、胰岛素样生长因子-I、前列腺素E2或血小板活化因子的浓度之间未发现相关性。然而，分别观察到ET-1-LI浓度与卵泡刺激素和IGF-II浓度之间存在显著正相关。这些数据表明，卵泡液中发现的高浓度ET-1可能在卵泡发育中发挥某些生理作用。