Merino S, Camprubi S, Tomás J M
Departmento de Microbiologia, Universidad de Barcelona, Spain.
J Diarrhoeal Dis Res. 1993 Mar;11(1):30-4.
A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for detection of Aeromonas hydrophila serogroup 0:34. The assay uses a detector antibody which shows no cross-reactions with Aeromonas strains not belonging to serogroup 0:34 or non-Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h; it is then microcentrifuged and the supernatant (unabsorbed antibody) titered on a microtiter plate coated with A. hydrophila cells from serogroup 0:34. All A. hydrophila strains from serogroup 0:34 that we tested in this manner reacted strongly with the detector antibody. Also, by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila 0:34 on different samples.
开发了一种用于检测嗜水气单胞菌0:34血清群的微量滴定板、抗体捕获酶联免疫吸附测定法。该测定法使用一种检测抗体，该抗体与不属于0:34血清群的气单胞菌菌株或非气单胞菌竞争生物无交叉反应。将检测抗体与样品混合并孵育1小时；然后进行微量离心，将上清液（未吸附的抗体）在包被有0:34血清群嗜水气单胞菌细胞的微量滴定板上进行滴定。我们以这种方式测试的所有0:34血清群嗜水气单胞菌菌株均与检测抗体发生强烈反应。此外，通过培养并用检测抗体进行免疫测定，我们确定并定量了不同样品上嗜水气单胞菌0:34的存在情况。
