Tsukamoto T, Takeda Y
Osaka Prefectural Institute of Public Health.
Kansenshogaku Zasshi. 1993 Apr;67(4):289-94. doi: 10.11150/kansenshogakuzasshi1970.67.289.
By using HeLa cells, we tried to detect enteroaggregative Escherichia coli from stock strains derived from infants with or without diarrhea in Brazil and Myanmer, and from diarrheal patients in Osaka, Japan. Enteroaggregative E. coli was detected from 7.9% in diarrheal cases, 5.6% in control cases in Brazil and from 5.4% in diarrheal cases, 3.4% in control cases in Myanmar. A total of 38 strains that showed aggregative adherence to HeLa cells was examined for colony hybridization with the 32P-labeled EAggEC probe. Thirty-four strains hybridized with the EAggEC probe, while 4 strains did not hybridize with the probe, suggesting an existence of another aggregative factor. Seventeen of the 38 strains were O-antigen serotypeable. Eight strains belonged to serogroups O44, O86, O111, O125 and O126, which have been considered to be enteropathogenic E. coli serogroups. Most of the 38 strains were H-antigen serotypeable.
我们利用HeLa细胞，试图从巴西和缅甸有或无腹泻症状的婴儿以及日本大阪腹泻患者的菌株中检测出肠集聚性大肠杆菌。在巴西，腹泻病例中肠集聚性大肠杆菌的检出率为7.9%，对照病例中为5.6%；在缅甸，腹泻病例中为5.4%，对照病例中为3.4%。对总共38株对HeLa细胞表现出集聚性黏附的菌株进行了用32P标记的EAggEC探针的菌落杂交检测。34株菌株与EAggEC探针杂交，而4株未与该探针杂交，这表明存在另一种集聚因子。38株菌株中有17株可进行O抗原血清分型。8株属于血清群O44、O86、O111、O125和O126，这些血清群被认为是肠致病性大肠杆菌血清群。38株菌株中的大多数可进行H抗原血清分型。
