巴西产肠致病性大肠杆菌（EPEC）中与抗菌药物耐药性相关的遗传元件。
Genetic elements associated with antimicrobial resistance in enteropathogenic Escherichia coli (EPEC) from Brazil.
机构信息
Departamento de Microbiologia, Universidade Federal de São Paulo, Rua Botucatu 862, São Paulo 04023-062, Brazil.
出版信息
BMC Microbiol. 2010 Jan 27;10:25. doi: 10.1186/1471-2180-10-25.
Abstract
BACKGROUND
We recently observed an association of resistance with a certain enteropathogenic Escherichia coli (EPEC) serotypes and identified a conjugative plasmid, similar to plasmid pED208, that was conserved among archival O111:H2/NM and O119:H2 strains of diverse geographical origin. In this study, we sought to determine the prevalence and distribution of this plasmid among a collection of EPEC isolates from Brazil, as well as to study the susceptibilities of these isolates to antimicrobial agents.
RESULTS
Resistance was more commonly seen in typical EPEC than atypical strains. The most prevalent resistances were to ampicillin, tetracycline, streptomycin and the sulfonamides. Markers for the EPEC conjugative multiresistance plasmid, were detected in 21 (30%) of typical but only 4 (5%) of atypical strains (p = 0.001, Chi-squared test). This plasmid, previously reported from only O111 and O119 strains was found in O55 and O127 strains and was associated with the presence of class 1 integrons.
CONCLUSION
Our data suggest a limited but expanding host range for the EPEC resistance plasmid.
摘要
背景
我们最近观察到某些肠致病性大肠杆菌（EPEC）血清型与耐药性有关，并鉴定出一种类似于质粒 pED208 的可转移性质粒，该质粒在不同地理来源的存档 O111：H2/NM 和 O119：H2 菌株中是保守的。在这项研究中，我们试图确定这种质粒在巴西收集的一组 EPEC 分离株中的流行程度和分布情况，以及研究这些分离株对抗菌药物的敏感性。
结果
典型 EPEC 菌株比非典型菌株更常见耐药性。最常见的耐药性是对氨苄西林、四环素、链霉素和磺胺类药物。在 21 株（30%）典型菌株而非 4 株（5%）非典型菌株中检测到 EPEC 可转移性多耐药质粒的标记（p = 0.001，卡方检验）。该质粒先前仅在 O111 和 O119 菌株中报道过，现在也在 O55 和 O127 菌株中发现，并与类 1 整合子的存在有关。
结论
我们的数据表明，EPEC 耐药质粒的宿主范围有限但在扩大。
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