Horikoshi Y, Ichikawa M, Tonouchi T, Mimaya J, Morita T, Ohtsuka Y, Kawabata H
Division of Hematology and Oncology, Shizuoka Children's Hospital.
Rinsho Ketsueki. 1993 May;34(5):620-7.
We amplified HIV-1 proviral DNA and RNA in serum by DNA-PCR and RT-RCR in 20 HIV-1 seropositive hemophiliacs asymptomatic carrier (AC): 10, AIDS-related complex (ARC):8, AIDS:2). HIV-1 DNA sequences were identified in 44 (93.6%) of 47 specimens. 23 (92.0%) of 25 samples in AC, 13 (100%) of 13 in ARC, 8 (88.8%) of 9 in AIDS had detectable bands. RNA sequences were identified in 36 (85.7%) of 42 specimens. 17 (70.8%) of 24 specimens in AC, 13 (100%) of 13 in ARC, 5 (100%) of 5 in AIDS cases had detectable bands. RNA in serum was strongly positive in two asymptomatic patients with progression to AIDS who showed no changes of other markers. Patients with almost normal CD4 cell counts had trace amounts of proviral DNA and RNA in serum. RNA in serum is the parameter most predictive of progression to AIDS and is detectable earlier than other parameters. From the results of RT-PCR before and after seroconversion, the serum of one patient was shown to be positive at 5 months before seroconversion.
我们采用DNA-PCR和RT-RCR方法，对20例HIV-1血清学阳性的无症状血友病携带者（AC，10例）、艾滋病相关综合征（ARC，8例）、艾滋病患者（2例）的血清中的HIV-1前病毒DNA和RNA进行了扩增。在47份标本中，44份（93.6%）检测到HIV-1 DNA序列。AC组25份样本中有23份（92.0%）、ARC组13份样本全部（100%）、艾滋病组9份样本中有8份（88.8%）出现可检测条带。在42份标本中，36份（85.7%）检测到RNA序列。AC组24份标本中有17份（70.8%）、ARC组13份样本全部（100%）、艾滋病组5份样本全部（100%）出现可检测条带。两名无症状患者血清中的RNA呈强阳性，随后进展为艾滋病，且其他标志物无变化。CD4细胞计数几乎正常的患者血清中前病毒DNA和RNA含量微量。血清中的RNA是预测进展为艾滋病最有效的指标，且比其他指标更早可检测到。根据血清转化前后RT-PCR的结果，一名患者在血清转化前5个月血清呈阳性。
