Kuriyama Y, Kawanishi Y, Kuge S, Iwase O, Uchida Y, Nakano M, Toyama K
First Department of Internal Medicine, Tokyo Medical College.
Rinsho Ketsueki. 1993 May;34(5):628-35.
In order to investigate the size variation of neoplastic cells from lymph nodes of 19 patients with B-cell non-Hodgkin's lymphomas (B-NHL), we have carried out the two-dimensional analysis by flow cytometry using the parameters of the forward light scatter (FLS) and the fluorescence intensity of surface immunoglobulin light chain (sIgL). Neoplastic B cells were identified as having the homogeneous characters of both FLS and sIgL (kappa or lambda) in comparison of normal B cells with the heterogeneity of counterpart sIgL (lambda or kappa). Then the size variation of neoplastic B cells was analyzed by regarding CD3+ T cells to a control scale. Consequently, the present procedures disclosed that B-NHL cases could be divided into five patterns from the view-point of the cell size and distribution. These results almost coincided with the cell types determined by the pathological diagnosis. Furthermore, it was also found that our data made it possible to classify small cleaved cell or large cell lymphomas into subtypes. The two-dimensional analysis by using the parameters of FLS and sIgL would be clinically useful for the rapid diagnosis of B-NHL and its malignant grade in addition to supporting pathological findings.
为了研究19例B细胞非霍奇金淋巴瘤（B-NHL）患者淋巴结中肿瘤细胞的大小变化，我们采用前向光散射（FLS）参数和表面免疫球蛋白轻链（sIgL）荧光强度，通过流式细胞术进行了二维分析。与正常B细胞表面免疫球蛋白轻链（sIgL）（λ或κ）的异质性相比，肿瘤性B细胞被鉴定为具有FLS和sIgL（κ或λ）的均一特征。然后，以CD3 + T细胞作为对照标准，分析肿瘤性B细胞的大小变化。因此，目前的方法表明，从细胞大小和分布的角度来看，B-NHL病例可分为五种模式。这些结果与病理诊断确定的细胞类型几乎一致。此外，还发现我们的数据能够将小裂细胞或大细胞淋巴瘤分为不同亚型。除了支持病理结果外，利用FLS和sIgL参数进行二维分析在临床上有助于B-NHL的快速诊断及其恶性程度判断。
