Kuriyama Y, Nakano M, Kawanishi Y, Toyama K
First Department of Internal Medicine, Tokyo Medical College.
Rinsho Ketsueki. 1995 Apr;36(4):279-85.
In order to investigate the size variation of neoplastic B cells (NBC) from lymph nodes of patients with B-cell lymphomas (BCL), we carried out two-dimensional analysis by flow cytometry (FCM) using the forward light scatter (FLS) and the fluorescence intensity (FI) of surface immunoglobulin light chain (sIgL). NBC were identified as having the homogeneous characteristics of both FLS and tumor-restricted sIgL (kappa or lambda). The FLS variation of NBC was then analyzed using CD3+ T-cells as a scale control. The results closely coincided with the cell types determined by the pathologic diagnosis. Secondly, we used FCM to investigate how strongly NBC express VLA-4 alpha and LFA-1 alpha, which seemed to play important roles in neoplastic follicle formation. The expression intensities of these molecules were classified into three grades (-, + & ++). The results were presented in the order of VLA-4 alpha and LFA-1 alpha; follicular 8/15 (++/++), 6/15 (++/+), 1/15 (+/++), partially follicular 3/5 (+/++), 2/5 (+/+), diffuse 2/16 (++/-), 6/16 (+/++), 8/16 (+/+), B-CLL 8/8 (-/+ or -). These findings suggest that follicular lymphomas are phenotypically distinguishable from diffuse lymphomas.
为了研究B细胞淋巴瘤（BCL）患者淋巴结中肿瘤性B细胞（NBC）的大小变化，我们使用前向光散射（FLS）和表面免疫球蛋白轻链（sIgL）的荧光强度（FI），通过流式细胞术（FCM）进行了二维分析。NBC被鉴定为具有FLS和肿瘤限制性sIgL（κ或λ）的均一特征。然后以CD3 + T细胞作为尺度对照，分析NBC的FLS变化。结果与病理诊断确定的细胞类型密切相符。其次，我们使用FCM研究NBC表达VLA-4α和LFA-1α的强度，这两种分子似乎在肿瘤性滤泡形成中起重要作用。这些分子的表达强度分为三个等级（-、+和++）。结果按VLA-4α和LFA-1α的顺序呈现；滤泡性8/15（++/++）、6/15（++/+）、1/15（+/++），部分滤泡性3/5（+/++）、2/5（+/+），弥漫性2/16（++/-）、6/16（+/++）、8/16（+/+），B细胞慢性淋巴细胞白血病8/8（-/+或-）。这些发现表明滤泡性淋巴瘤在表型上可与弥漫性淋巴瘤区分开来。
