烟酰胺腺嘌呤二核苷酸对大鼠肝细胞核中Ca2+转运系统的影响：NAD+刺激Ca2+释放
Effect of nicotinamide-adenine dinucleotides on Ca2+ transport system in rat liver nuclei: stimulation of Ca2+ release by NAD+.
作者信息
Oishi K, Yamaguchi M
机构信息
Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.
出版信息
Mol Cell Biochem. 1993 Apr 21;121(2):127-33. doi: 10.1007/BF00925971.
The effect of nicotinamide-adenine dinucleotides (NAD+ and NADP+) on Ca2+ transport in rat liver nuclei was investigated. Ca2+ uptake and release were determined with a Ca2+ electrode. Ca2+ uptake was dependent on adenosine triphosphate (ATP; 2 mM). The presence of NAD+ (2 mM) or NADP+ (1 and 2 mM) caused a significant inhibition of Ca2+ uptake following addition of 2 mM ATP. Ca2+, which accumulated in the nuclei during 6 min after ATP addition, was significantly released by the addition of NAD+ (0.5-2 mM) or NADP+ (0.1-2 mM). However, the effect of NADH (2 mM) or NADPH (2 mM) on Ca2+ uptake and release clearly weakened in comparison with the effects of NAD+ and NADP+. Meanwhile, ryanodine (10 microM), thapsigargin (10 microM) or oxalate (0.5 mM) had no effect on Ca2+ uptake and release in rat liver nuclei. These reagents did not significantly alter the effects of 2 mM NAD+ on Ca2+ uptake and release. Thus, NAD+ and NADP+ had a potent effect on Ca2+ transport in rat liver nuclei. The present findings suggest that the liver cytosolic NAD+ (NADP+) is a factor in the regulation of the nuclear Ca2+ concentration.
研究了烟酰胺腺嘌呤二核苷酸（NAD⁺和NADP⁺）对大鼠肝细胞核中Ca²⁺转运的影响。用Ca²⁺电极测定Ca²⁺的摄取和释放。Ca²⁺摄取依赖于三磷酸腺苷（ATP；2 mM）。加入2 mM ATP后，NAD⁺（2 mM）或NADP⁺（1和2 mM）的存在会显著抑制Ca²⁺摄取。在加入ATP后6分钟内积累在细胞核中的Ca²⁺，会因加入NAD⁺（0.5 - 2 mM）或NADP⁺（0.1 - 2 mM）而显著释放。然而，与NAD⁺和NADP⁺的作用相比，NADH（2 mM）或NADPH（2 mM）对Ca²⁺摄取和释放的作用明显减弱。同时，ryanodine（10 μM）、thapsigargin（10 μM）或草酸盐（0.5 mM）对大鼠肝细胞核中Ca²⁺的摄取和释放没有影响。这些试剂没有显著改变2 mM NAD⁺对Ca²⁺摄取和释放的作用。因此，NAD⁺和NADP⁺对大鼠肝细胞核中的Ca²⁺转运有显著作用。目前的研究结果表明，肝细胞质中的NAD⁺（NADP⁺）是调节细胞核Ca²⁺浓度的一个因素。
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