核Ca2+摄取抑制剂对大鼠肝细胞核中Ca(2+)激活的DNA片段化的影响。

Effect of nuclear Ca2+ uptake inhibitors on Ca(2+)-activated DNA fragmentation in rat liver nuclei.

作者信息

Yamaguchi M, Oishi K

机构信息

Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.

出版信息

Mol Cell Biochem. 1995 Jul 5;148(1):33-7. doi: 10.1007/BF00929500.

DOI:10.1007/BF00929500

PMID:7476931

Abstract

The effect of nuclear Ca2+ uptake inhibitors on the Ca(2+)-activated DNA fragmentation in rat liver nuclei was investigated. The addition of Ca2+ (40 microM) into the reaction mixture containing liver nuclei in the presence of 2.0 mM ATP caused a remarkable increase in nuclear DNA fragmentation. This Ca(2+)-activated DNA fragmentation was not seen in the absence of ATP, because nuclear Ca2+ uptake is not initiated without ATP addition. Moreover, the presence of various reagents (10 microM arachidonic acid, 2.0 mM NAD+, 10 microM zinc sulfate and 0.2 mM N-ethylmaleimide), which could inhibit Ca(2+)-ATPase activity and Ca2+ uptake in the nuclei, produced a significant inhibition of the Ca(2+)-activated DNA fragmentation in the nuclei. The results show that the Ca(2+)-activated DNA fragmentation is involved in the uptake of Ca2+ by the nuclei, suggesting a role of Ca2+ transport system in the regulation of liver nuclear functions.

摘要

研究了核Ca2+摄取抑制剂对大鼠肝细胞核中Ca(2+)激活的DNA片段化的影响。在含有2.0 mM ATP的反应混合物中向肝细胞核添加Ca2+(40 microM)会导致核DNA片段化显著增加。在没有ATP的情况下未观察到这种Ca(2+)激活的DNA片段化，因为不添加ATP就不会启动核Ca2+摄取。此外，各种能够抑制核中Ca(2+)-ATP酶活性和Ca2+摄取的试剂(10 microM花生四烯酸、2.0 mM NAD+、10 microM硫酸锌和0.2 mM N-乙基马来酰亚胺)的存在对核中Ca(2+)激活的DNA片段化产生了显著抑制作用。结果表明，Ca(2+)激活的DNA片段化与核摄取Ca2+有关，提示Ca2+转运系统在肝细胞核功能调节中起作用。