Legorreta-Herrera M, Fiallos-Leon C, Cedillo-Barron L, Martinez-Gomez F, Foster-Cuevas M, Favila-Castillo L
Department of Immunology, National School of Biological Sciences, Mexico DF, Mexico.
Parasite Immunol. 1993 Mar;15(3):143-51. doi: 10.1111/j.1365-3024.1993.tb00594.x.
The transfer of spleen cells from (BALB/c x C57Bl/6) F1 mice recovered from a Plasmodium chabaudi chabaudi AS infection into irradiated syngeneic recipients conferred protection. Neither elimination of Thy-1+ cells nor in vitro irradiation of immune cells before transfer affected protection while both anti-Thy-1 treatment and irradiation abolished the appearance of anti-P. c. chabaudi antibodies in the recipients. Superinfection of immune spleen cell donors did not improve their capability to transfer protection which was also unaffected by anti-Thy-1 treatment. The serum of mice after one infection was only marginally protective when transferred into irradiated recipients and a second infection improved the protective activity of serum which was not further improved by six infections. The co-transfer of immune serum and immune cells did not result in any synergistic effect. On the other hand, when P. c. chabaudi AS (BALB/c x C57Bl/6) F1 infected mice were challenged with a high dose of Plasmodium yoelii 17XL at crisis, the mice were unable to control the heterologous parasite. When mice were challenged with P. yoelii 17XL several weeks after infection with P. c. chabaudi AS, a good degree of cross-protection was observed.
将从感染恰氏疟原虫阿氏亚种（Plasmodium chabaudi chabaudi AS）中恢复的（BALB/c×C57Bl/6）F1小鼠的脾细胞转移至经照射的同基因受体中可提供保护。在转移前消除Thy-1+细胞或对免疫细胞进行体外照射均不影响保护作用，而抗Thy-1处理和照射均消除了受体中抗恰氏疟原虫抗体的出现。对免疫脾细胞供体进行再次感染并不能提高其转移保护的能力，且抗Thy-1处理对此也无影响。单次感染后的小鼠血清转移至经照射的受体中时仅有微弱的保护作用，二次感染可提高血清的保护活性，六次感染也未进一步提高该活性。免疫血清和免疫细胞的共同转移未产生任何协同效应。另一方面，当恰氏疟原虫阿氏亚种（BALB/c×C57Bl/6）F1感染的小鼠在病情危急时用高剂量约氏疟原虫17XL（Plasmodium yoelii 17XL）攻击时，小鼠无法控制异源寄生虫。当小鼠在感染恰氏疟原虫阿氏亚种数周后用约氏疟原虫17XL攻击时，观察到了良好程度的交叉保护作用。
