Shaposhnikova V V, Dobrovinskaya O R, Eidus L Kh
Institute of Theoretical and Experimental Biophysics, Acad. Sci. Russia, Pushchino, Moscow Region.
Radiat Res. 1993 Jun;134(3):301-6.
The effect of the interaction of different types of cells on the interphase death and pycnosis of thymocytes irradiated in vitro was studied. When removed from the thymus suspension of cells with natural killer activity, medullary thymocytes and macrophages did not change the radiation-induced death of cortical thymocytes. On the other hand, postirradiation incubation of cortical thymocytes together with unirradiated thymocytes or with cells of certain other cell lines diminished thymocyte death. Mixing the cell suspensions and changing the incubation medium decreased thymocyte death. All of these results indicate that these cells produce soluble mediators that are toxic to the cells that secrete them. The possible nature of these autotoxic mediators has been studied using inhibitors of arachidonic acid metabolism. Inhibitors of phospholipase A2 or lipoxygenase reduced interphase death markedly, while an inhibitor of cyclooxygenase did not. These data suggest that some lipoxygenase products may serve as autotoxic mediators in the interphase death of thymocytes.
研究了不同类型细胞相互作用对体外照射胸腺细胞间期死亡和固缩的影响。当从具有自然杀伤活性的细胞、髓质胸腺细胞和巨噬细胞的胸腺悬液中去除时，并未改变皮质胸腺细胞的辐射诱导死亡。另一方面，皮质胸腺细胞与未照射的胸腺细胞或某些其他细胞系的细胞一起进行照射后孵育，可减少胸腺细胞死亡。混合细胞悬液并更换孵育培养基可降低胸腺细胞死亡。所有这些结果表明，这些细胞产生对分泌它们的细胞有毒的可溶性介质。已使用花生四烯酸代谢抑制剂研究了这些自毒介质的可能性质。磷脂酶A2或脂氧合酶抑制剂可显著降低间期死亡，而环氧化酶抑制剂则无此作用。这些数据表明，某些脂氧合酶产物可能作为胸腺细胞间期死亡中的自毒介质。
