Yamada T, Ohyama H
Scan Electron Microsc. 1981;4:55-60.
Thymocytes are radiosensitive and show interphase death (immediate death) after irradiation. The present investigation was undertaken to characterize by scanning electron microscopy the shape of thymocytes irradiated in vitro with 1 kR X-rays and determine the cell surface changes that accompany the development of interphase death. Exposing the cells to X-rays caused disappearance of the microvilli, usually observed with viable cells. Association of dead thymocytes with porous surface without any digitations was confirmed by the examination of enriched dead cell populations prepared by centrifuging the cell suspension in Percoll density gradient.
胸腺细胞对辐射敏感，照射后会出现间期死亡（即刻死亡）。本研究旨在通过扫描电子显微镜对体外接受1千伦琴X射线照射的胸腺细胞形态进行表征，并确定伴随间期死亡发生的细胞表面变化。将细胞暴露于X射线下会导致微绒毛消失，而微绒毛通常在活细胞中可见。通过检查经Percoll密度梯度离心细胞悬液制备的富集死细胞群体，证实了死亡的胸腺细胞与无任何指状突起的多孔表面相关联。
