Maggi C A, Lecci A, Santicioli P, Del Bianco E, Giuliani S
Pharmacol. Department, A Menarini Pharmaceuticals, Florence, Italy.
Agents Actions. 1993;38 Spec No:C28-30. doi: 10.1007/BF01991127.
The involvement of capsaicin-sensitive primary afferent neurons has been investigated in a rat model of cyclophosphamide (CYP)-induced cystitis. CYP (150 mg/kg i.p., 48 h before) was administered in both vehicle- and capsaicin-(50 mg/kg s.c., 4 days before)-treated rats. Some experiments were performed 96 h after bilateral removal of pelvic ganglia (bladder denervation). CYP produced a marked detrusor hyperreflexia which was abolished by capsaicin pretreatment. CYP produced a marked increase in bladder weight and plasma protein extravasation (PPE, measured by Evans blue leakage technique): CYP-induced PPE was reduced by bladder denervation and was aggravated by capsaicin pretreatment. PPE aggravation by capsaicin was abolished by ganglionectomy. The bladder content of calcitonin gene-related peptide, was unaffected CYP. We conclude that CYP-induced decrease in bladder capacity is entirely mediated through stimulation of capsaicin-sensitive bladder afferents. At the time chosen as end point in these experiments, capsaicin-sensitive afferents exert an antiinflammatory influence on CYP-induced cystitis.
在环磷酰胺（CYP）诱导的膀胱炎大鼠模型中，研究了辣椒素敏感的初级传入神经元的参与情况。在给予载体处理和辣椒素（50mg/kg皮下注射，4天前）处理的大鼠中，均腹腔注射CYP（150mg/kg，48小时前）。在双侧切除盆腔神经节（膀胱去神经支配）96小时后进行了一些实验。CYP产生了明显的逼尿肌反射亢进，而辣椒素预处理可消除这种亢进。CYP使膀胱重量和血浆蛋白外渗（通过伊文思蓝渗漏技术测量的PPE）显著增加：CYP诱导的PPE在膀胱去神经支配后降低，而在辣椒素预处理后加重。辣椒素引起的PPE加重在神经节切除后消除。降钙素基因相关肽的膀胱含量不受CYP影响。我们得出结论，CYP诱导的膀胱容量降低完全是通过刺激辣椒素敏感的膀胱传入神经介导的。在这些实验中选为终点的时间点，辣椒素敏感的传入神经对CYP诱导的膀胱炎发挥抗炎作用。
