Kojima M, Sasaki K
Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan.
Nippon Ganka Gakkai Zasshi. 1993 Mar;97(3):324-32.
In order to find an appropriate model of experimentally induced streptozotocin diabetic cataract rat for evaluation of the anti-cataract agents. Experimental diabetic cataracts were induced in 3 rat strains (Sprague-Dawley SD, Brown Norway BN, Wistar WIS), age 5 and 7 weeks old, by intravenous injection of 70 mg/kg streptozotocin. The cataract progression was followed by morphological and biochemical assessment. Two types of initial cataractous change were observed in SD and WIS rats which mainly consisted of water-vacuole formation in the peripheral area and a diffuse cloudiness progressing around the anterior Y-suture area. The initial change of BN rats was a Y-suture dissociation and fine water-clefts with dot-like vacuoles. Those initial changes in SD and WIS rats differed from those in BN. No difference in aldose reductase activities among the three strains was found, but BN rats had significantly higher sorbitol dehydrogenase activity than SD or WIS rats. It seems that 7-week-old BN rats are more suitable for experimental diabetic cataract models than SD or WIS.
为了找到一种合适的实验性诱导链脲佐菌素糖尿病性白内障大鼠模型来评估抗白内障药物。对3种品系（斯普拉格-道利大鼠SD、挪威棕大鼠BN、Wistar大鼠WIS）、年龄为5周和7周的大鼠，通过静脉注射70mg/kg链脲佐菌素诱导实验性糖尿病性白内障。通过形态学和生化评估追踪白内障的进展。在SD和WIS大鼠中观察到两种类型的初始白内障变化，主要包括周边区域水液泡形成以及围绕前Y缝区域的弥漫性混浊进展。BN大鼠的初始变化是Y缝分离以及带有点状液泡的细微水隙。SD和WIS大鼠的那些初始变化与BN大鼠不同。在这三个品系中未发现醛糖还原酶活性有差异，但BN大鼠的山梨醇脱氢酶活性显著高于SD或WIS大鼠。似乎7周龄的BN大鼠比SD或WIS大鼠更适合作为实验性糖尿病性白内障模型。
