Cross-reactivity of IgM- and IgG-secreting B cells in autoimmune mice.
作者信息
Ishigatsubo Y, Igarashi T, Ohno S, Ueda A, Okubo T, Klinman D M
机构信息
First Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
出版信息
Arthritis Rheum. 1993 Jul;36(7):1003-6. doi: 10.1002/art.1780360718.
OBJECTIVE
To determine the frequency with which in vivo-activated B cells secrete cross-reactive antibodies in lupus-prone mice.
METHODS
Analysis of freshly isolated splenic lymphocytes by chamber enzyme-linked immunospot assay.
RESULTS
Young New Zealand black, New Zealand black x New Zealand white, and MRL/lpr mice expressed repertoires in which 16-20% of IgM-secreting cells and 0-2% of IgG-secreting cells were cross-reactive. By comparison, 21-31% of IgM- and 4-14% of IgG-secreting cells in adult animals with active lupus were cross-reactive (P < 0.01).
CONCLUSION
Cross-reactive B cells constitute an abnormally large proportion of the repertoire expressed in mice with active autoimmune disease.
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