未感染的献血者在蛋白质印迹法中出现的HIV-1糖蛋白反应性。
Apparent HIV-1 glycoprotein reactivity on western blot in uninfected blood donors.
作者信息
Healey D S, Bolton W V
机构信息
National HIV Reference Laboratory, Fairfield Hospital, Victoria, Australia.
出版信息
AIDS. 1993 May;7(5):655-8. doi: 10.1097/00002030-199305000-00007.
OBJECTIVES
To investigate samples with 'false-positive' reactivity to HIV-1 glycoproteins on Western blot (WB).
DESIGN
Samples from 13 blood donors with glycoprotein reactivity were examined for serological evidence of HIV infection and followed-up where possible.
METHODS
Samples were tested for anti-HIV-1, HIV-1 p24 antigen, anti-HIV-2 and anti-HTLV-1. Reactivity to multimeric, monomeric, and deglycosylated gp41 was determined, as was the ability of recombinant gp160 (rgp160) to inhibit reactivity to multimeric gp41.
RESULTS
Serology and follow-up failed to confirm HIV infection in any of the donors. All samples reacted to multimeric gp41, and eight out of the 13 reacted to deglycosylated gp41. Reactivity on a commercial WB was inhibited by rgp160.
CONCLUSION
Apparent reactivity to HIV-1 glycoprotein may occur in individuals with no other serological evidence of HIV infection. Reactivity to different forms of gp41 and inhibition by rgp160 suggested that the observed WB reactivity may be due to cross-reactivity with gp41 rather than to a co-migrating contaminant.
目的
调查在蛋白质印迹法（WB）中对HIV-1糖蛋白呈“假阳性”反应性的样本。
设计
对13名具有糖蛋白反应性的献血者的样本进行检测，以寻找HIV感染的血清学证据，并在可能的情况下进行随访。
方法
对样本进行抗HIV-1、HIV-1 p24抗原、抗HIV-2和抗HTLV-1检测。测定对多聚体、单体和去糖基化gp41的反应性，以及重组gp160（rgp160）抑制对多聚体gp41反应性的能力。
结果
血清学检测和随访未能证实任何一名献血者感染HIV。所有样本均对多聚体gp41有反应，13份样本中有8份对去糖基化gp41有反应。rgp160可抑制商用WB上的反应性。
结论
在没有其他HIV感染血清学证据的个体中，可能会出现对HIV-1糖蛋白的明显反应性。对不同形式gp41的反应性以及rgp160的抑制作用表明，观察到的WB反应性可能是由于与gp41的交叉反应，而不是由于共迁移污染物。
Zotero 插件