Imai K, Fukushima T, Uzu S
Branch Hospital Pharmacy, University of Tokyo, Japan.
Biomed Chromatogr. 1993 May-Jun;7(3):177-8. doi: 10.1002/bmc.1130070316.
Some L- and D-amino acids (Phe or Leu) were derivatized with a fluorogenic reagent, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a Pirkle-type column, Sumichiral OA 2500(S) [(S)-1-naphthylglycyl-3,5-dinitrophenylamide silica gel] with a mobile phase of 20 mM ammonium acetate in methanol. The fluorometric detection was made at 530 nm with excitation at 470 nm. No racemization of the enantiomers occurred during the derivatization reaction. The separation factors (alpha) for NBD-L-Phe and NBD-D-Phe, and NBD-L-Leu and NBD-D-Leu, were 1.27 and 1.17, respectively. The detection limits were in the range of ca. 30 fmol.
一些L-和D-氨基酸（苯丙氨酸或亮氨酸）用荧光试剂4-氟-7-硝基-2,1,3-苯并恶二唑（NBD-F）进行衍生化，并在Pirkle型柱Sumichiral OA 2500(S) [（S）-1-萘基甘氨酰-3,5-二硝基苯甲酰胺硅胶]上分离，流动相为甲醇中的20 mM醋酸铵。荧光检测在530 nm处进行，激发波长为470 nm。在衍生化反应过程中，对映体未发生消旋化。NBD-L-苯丙氨酸与NBD-D-苯丙氨酸以及NBD-L-亮氨酸与NBD-D-亮氨酸的分离因子（α）分别为1.27和1.17。检测限约为30 fmol。
