Kato M, Fukushima T, Santa T, Homma H, Imai K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Biomed Chromatogr. 1995 Jul-Aug;9(4):193-4. doi: 10.1002/bmc.1130090409.
The concentrations of D-amino acids and their enantiomeric ratios in wind samples were determined by high-performance liquid chromatography with fluorometric detection. We used Pirkle type chiral stationary phases and fluorogenic reagent, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) for simplicity and high sensitivity. The amino acids determined were D-enantiomers of alanine (Ala), aspartic acid, glutamic acid, isoleucine (Ile) and leucine (Leu), D-Asparagine, -glutamine and -lysine were not detected. D-Leu was detected in red and rosé wine samples, and D-Ile was determined only in rosé wine. In contrast, neither D-Leu nor Ile were detected in white wine samples. The concentration of D-Ala was the most prominent among these amino acids with the highest content of approximately 180 microns (D/D + L ratio; 25%) in rosé wine.
通过高效液相色谱荧光检测法测定了风样中D-氨基酸的浓度及其对映体比例。为了简便和高灵敏度，我们使用了Pirkle型手性固定相和荧光试剂4-氟-7-硝基-2,1,3-苯并恶二唑（NBD-F）。所测定的氨基酸为丙氨酸（Ala）、天冬氨酸、谷氨酸、异亮氨酸（Ile）和亮氨酸（Leu）的D-对映体，未检测到D-天冬酰胺、-谷氨酰胺和-赖氨酸。在红葡萄酒和桃红葡萄酒样品中检测到了D-亮氨酸，仅在桃红葡萄酒中测定到了D-异亮氨酸。相比之下，白葡萄酒样品中未检测到D-亮氨酸和D-异亮氨酸。在这些氨基酸中，D-丙氨酸的浓度最为显著，在桃红葡萄酒中的含量最高，约为180微米（D/D + L比例；25%）。
