Feldsine P T, Falbo-Nelson M T, Hustead D L
BioControl Systems, Inc., Bothell, WA 98011.
J AOAC Int. 1993 May-Jun;76(3):694-7.
A new enzyme immunoassay (EIA) method for detection of motile and non-motile Salmonella was examined in a comparative study. This method uses a proprietary formulation of polyclonal antibodies to Salmonella and is controlled to maintain specificity. Sensitivity is enhanced with an additional antibody reaction designed to minimize false-negative reactions attributable to steric interference that can occur during conjugate binding in immunoassay procedures. Twenty food types representative of a wide variety of food products were analyzed by both the EIA method and the AOAC/Bacteriological Analytical Manual (BAM) method, 967.26. Of the 1000 samples analyzed, there was a 95.6% agreement rate between the EIA method and the AOAC/BAM method. False-negative rates for the 2 methods were comparable for all foods and all Salmonella levels except ground poultry, where the EIA method detected significantly more confirmed positive samples than did the AOAC/BAM method. Twenty-seven samples were positive by EIA but negative by the culture method, and 17 samples were negative by EIA but positive by the culture method. There were no false-positive isolates detected in the comparative study.
在一项对比研究中，对一种用于检测活动型和非活动型沙门氏菌的新型酶免疫测定（EIA）方法进行了检验。该方法使用一种针对沙门氏菌的多克隆抗体专有制剂，并进行控制以保持特异性。通过额外的抗体反应增强了灵敏度，该反应旨在最大程度减少免疫测定程序中缀合物结合期间可能因空间位阻干扰而导致的假阴性反应。采用EIA方法和《美国官方分析化学师协会/细菌学分析手册》（AOAC/BAM）方法967.26对代表多种食品的20种食品类型进行了分析。在分析的1000个样本中，EIA方法与AOAC/BAM方法之间的一致率为95.6%。除了碎禽肉外，两种方法对所有食品和所有沙门氏菌水平的假阴性率相当，在碎禽肉检测中，EIA方法检测到的确诊阳性样本明显多于AOAC/BAM方法。27个样本EIA检测为阳性但培养法检测为阴性，17个样本EIA检测为阴性但培养法检测为阳性。在对比研究中未检测到假阳性分离株。
